FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3069721
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14150.045.7982068
GTCTCGC13100.038.7302061
TCGCTCT14700.035.2677354
CTGTCGC18100.035.00289
GTCTTGC30950.032.7861481
GTATTAT7600.032.425631
CTCGCTC17200.028.048443
GTGTTAG20500.027.9316311
GTATTAG10200.027.7132511
CGCTCTG19050.027.214475
GTGTTAT12350.026.9969021
TCTCGCT18050.026.7926372
GTATAAC5350.025.7408491
GTGTAAA21100.025.4198111
GTGTTAC14000.025.3682841
TAATACT9750.025.1094194
GTATTAC7050.024.674321
GTATAAT8400.024.160271
TTGCTCT41850.024.0876794
GTTAGCC17100.024.0016543
Produced by FastQC (version 0.11.5)