FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2813886
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11150.042.618488
GTCTCGC13200.036.134551
CTGTCGC15400.030.856899
GTCTTGC26900.030.3583951
TCTCGCT15650.028.561792
TCGCTCT15200.028.4253644
GTATAGG6900.028.2792151
CTCGCTC16050.027.392983
GTACTAT7350.025.5645771
GTATTAC6100.024.8795241
GTATTAG9600.024.08971
ACCGTGT9950.023.87928
CGCTCTG16150.023.6278675
TTGCTCT36300.023.6068884
GTATTAT8650.023.3934671
GTCTCAC32550.023.3126131
GTGTAAT10650.023.0718251
GTGTAGA14450.023.0060791
GTATTAA9150.022.904961
GTATATA12800.022.0194931
Produced by FastQC (version 0.11.5)