FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3979385
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17950.035.297768
GTCTTGC36200.031.140851
GTCTCGC18000.030.5109351
GTATTAG15350.029.6583251
CTGTCGC22450.028.2224859
TCGCTCT20500.027.048444
GTGTAAA25700.026.1495551
GTGTTAG28000.025.5500431
GTATTAT14950.024.6515051
GTTTCAC61950.024.1981583
GTCTTAC16450.023.7215081
TCTCGCT22650.023.55112
CTCGCTC21450.023.5196593
GTATAAT13050.023.2569751
GTGTTAC16500.022.7737141
GGGTTTC69300.022.6277281
GTACTAT10250.022.5600781
TTGCTCT52450.022.1047554
CGCTCTG22700.021.5678355
GTATAAG12400.021.5622711
Produced by FastQC (version 0.11.5)