FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3680141
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15450.035.8841138
GTCTCGC16150.034.112381
GTCTTGC34050.030.868951
TCGCTCT17400.030.2074174
CTGTCGC19600.029.3858689
GTCTTAC15050.028.417541
CGCTCTG18850.026.3558945
CTCGCTC20650.024.7558773
GTGTTAC15550.024.240631
TCTCGCT21350.023.666782
GTCTCAC45500.023.2601471
GTACGAT2903.2958997E-722.4965271
GGTATAC7600.021.9374031
TACACTG23900.021.690755
GTGTTAG25750.020.5502241
GTTTCAC60050.020.3833663
TTGCTCT51950.020.3737874
GTACTAC7150.020.2765881
GTATTAT11150.020.153841
GGAGTGC85750.020.151955
Produced by FastQC (version 0.11.5)