FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3550882
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16200.046.2180028
CTGTCGC20900.035.8244829
GTCTCGC17150.032.4529081
GTCTTGC35150.030.845581
GTATAAT11250.030.197591
TCGCTCT16800.030.0027544
GTATTAG10750.028.2402041
GTCGTAA1907.3320753E-626.6300161
GTATAGA10950.025.7440851
GTATTAT10650.025.1118721
GTATAGT10250.024.6814771
GTGTTAG25200.024.3806531
TCTCGCT19650.024.2154372
CTCGCTC19950.023.4826283
GTCTTAC14200.023.4151231
GTGTTAC16600.023.077821
CGCTCTG20700.022.9541025
TTGCTCT47750.022.9214744
TAGTACT9400.022.2148044
GTTAGCC20450.021.8511663
Produced by FastQC (version 0.11.5)