FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3550882
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14550.039.0946588
GTCTCGC15350.034.4928551
GTCTTGC31650.030.9367561
TCGCTCT16200.030.223674
CTGTCGC20950.029.2092849
TCTCGCT19500.027.4004042
CTCGCTC19500.026.9551373
ATAGTAC8350.025.8695033
GTCTTAC13900.024.5243631
GTATTAC9350.024.0472131
GTATTAG12800.023.7987421
GTATAAT10600.023.264181
GTACTAC6900.023.1253471
CGTTTCG2202.3554205E-522.9066419
GTCCTAC10650.022.473931
GTACTAG8100.022.3856491
GTGTAAC13050.022.2312641
GGAGTGC75400.022.1549075
TTGCTCT47050.021.7311154
GTCCGGT2353.7009715E-521.604531
Produced by FastQC (version 0.11.5)