FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3239476
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC15250.048.6429821
TCTGTCG12900.045.7659958
CTGTCGC16050.042.1668939
TCGCTCT17150.038.2028734
TCTCGCT18650.036.6744422
GTCTTGC29600.033.333561
CTCGCTC20050.033.036363
CGCTCTG19400.031.5453935
GGGTTTC53050.025.2511061
GTGTTAG23250.024.781061
GTCTCAC38750.024.7191091
GTATAGA10200.024.712731
GTCTTAC12250.024.1046371
GTATTAG11750.023.9044951
GTTTCAC49800.023.565523
GTACTAA6450.023.4483571
GTCTAAT8650.023.3128191
TTGCTCT41050.023.1515224
TACACCG2802.5087502E-723.1421265
CTGTTGC34450.022.9891139
Produced by FastQC (version 0.11.5)