FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3239476
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14000.038.577848
GTCTTGC30450.038.3407861
GTCTCGC14650.036.402181
TCCGTCG1251.3258996E-534.5657468
CTGTCGC18350.032.9600269
TCGCTCT16700.031.9080164
TCTCGCT18750.028.0414222
GTATTAG10200.027.554831
TTGCTCT41500.027.4153334
CTCGCTC17800.026.699833
CGCTCTG17350.026.563085
GGGTTTC56400.026.0665991
CTTGCTC41800.025.3236083
TCTGTCA41600.025.1003258
TACCCGT3501.4188117E-1024.6894365
GTATTAT9950.024.6257321
GTTTCAC51050.024.5435893
GTGTTAG21200.024.1354731
GTCTTAC12450.023.7327351
GTATAAT9450.023.6408581
Produced by FastQC (version 0.11.5)