FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3686103
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15350.044.5589378
GTCTCGC17950.041.7259561
TCGCTCT19800.037.08984
CTGTCGC19250.035.531419
GTCTTGC34550.033.9763641
TCTCGCT20950.032.991862
CTCGCTC21900.032.5469743
CGCTCTG23350.029.6008345
GTTTCAC53400.028.3137633
GGGTTTC61400.027.6809541
GTCTTAC15150.027.0956541
GTATTAG12750.026.5475881
GTATTAT10650.026.3725681
TCGTGCG1400.001041353125.713529
GTGTTAG25300.025.334181
TTGCTCT46350.025.1642994
GTATAAG10150.024.8335841
GTATTAC8150.023.8585221
CTTGCTC44350.023.3769823
TTACACT15850.023.1665044
Produced by FastQC (version 0.11.5)