FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2802494
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12950.047.2579738
CTGTCGC15350.042.216619
TCGCTCT14150.040.1972734
GTCTCGC14300.038.8323861
CGCTCTG14950.035.6382565
GTCTTGC26250.035.1657641
CTCGCTC16400.032.4873123
TCTCGCT17500.032.092092
GTATTAA9450.030.5258351
GTCTTAC10250.028.1433321
GTATAGG7050.027.6193891
GGTATAC5300.027.2140711
GTACCGT1907.4619966E-626.5695271
GTATACG2456.7864676E-826.4920641
TTGCTCT33500.026.4354214
GGGTTTC44100.026.001471
GTATCGG1400.001031270125.7561741
CTTGCTC33400.025.436743
GTATAAG9150.025.2213461
GTTTCAC41150.025.0202793
Produced by FastQC (version 0.11.5)