FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3078455
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14000.048.3416988
GTCTCGC14900.047.3699531
CTGTCGC15250.043.4350249
TCGCTCT16500.041.01724
TCTCGCT17500.036.6162642
CGCTCTG18100.035.8002475
CTCGCTC18350.034.9201433
GTCTTGC28750.033.3178521
GTTACCC11250.026.239373
GTGTTAC13250.026.0908681
GTTTCAC44400.025.9453223
GGGTTTC50000.025.4956681
TAATACT8250.025.3084854
CTTGCTC37100.025.2285043
TTGCTCT38250.025.2229234
TTCACCG8000.025.1993925
GTGTAAG15750.024.6931421
GTATTAG10800.024.0072211
ACCGTGT9350.023.8710848
GTCTCAC37300.023.1705071
Produced by FastQC (version 0.11.5)