FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3078455
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13700.035.2162448
GTCTTGC27550.034.5331651
GTCTCGC12500.034.0193521
CTGTCGC16750.030.0895449
TCGCTCT15350.027.6769544
TCTCGCT15100.026.2328262
GTATTAT9000.025.626631
GCGTAGT3409.640644E-1125.4381981
TCTGTCA37050.025.0720758
TTGCTCT41400.024.002334
GTACTAT7250.023.8592761
CGCTCTG16450.023.6375565
CTCGCTC16750.023.2141973
GTACGGA2802.482866E-723.1669331
GTGTAAG16300.022.993221
GTCTCAC37950.022.7904821
GGGTTTC48900.022.6984351
GTTAGCC15900.022.6437073
GTTTCAC44550.022.6284623
CTTGCTC39550.022.3940853
Produced by FastQC (version 0.11.5)