FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2995503
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14150.046.3463631
GTCTTGC26200.038.7836381
TCGCTCT16300.037.9902044
GTATCGG1157.448036E-637.5996781
TCTGTCG13000.036.0047538
TCTCGCT17200.035.173892
CGCTCTG16250.033.676045
CTCGCTC17550.033.643223
CTGTCGC16200.030.6681529
TTGCTCT37100.026.0070844
GGGTTTC46300.025.9935861
GTATAAG7350.025.492751
GTGTTAA10200.025.4350761
GTGTTAG19300.025.3911461
GTTTCAC42800.024.7305833
TAATCCG1751.3164047E-424.6873235
GTATAAC5600.024.450981
CTTGCTC38100.024.3795413
GTACTAC5950.024.223881
GTCTCAC35350.024.0559921
Produced by FastQC (version 0.11.5)