FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2923256
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12950.048.926028
CTGTCGC14300.046.824589
GTCTTGC27200.036.7999461
GTCTCGC13750.035.612911
TCGCTCT15100.032.900244
TCTCGCT17150.028.9675622
CGCTCTG17300.027.0516785
CTCGCTC17150.026.8684643
GTTTCAC45100.026.6604163
GTGTTAG20500.026.3456341
GGGTTTC49450.025.7755681
GTCTCAC34450.025.5018621
GTTAGCC16750.025.3608723
GTATTAT8000.025.203991
GGTATAC6000.025.203991
TTGCTCT37250.025.1271974
GTATTAG8650.024.9750521
GTCTTAC11300.024.8534931
GTGTTAC11750.024.5145191
GTGTAGT16150.024.0781171
Produced by FastQC (version 0.11.5)