FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2796099
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11550.043.6434638
GTCTTGC27900.035.3814771
TAGCGCG850.002837448833.882419
CTGTCGC15900.033.0566649
GTCTCGC12200.032.483471
TCGCTCT13300.031.4019184
CTCGCTC15500.027.8740063
GTCTTAC10900.027.764021
CTTGTCG2705.999027E-926.6667149
CGCTCTG15200.026.0554715
TCTCGCT15700.025.6916832
GTATTAG8500.025.430911
GTATAGA9400.025.2956391
TTGCTCT36050.025.1677954
GTGTTAT9800.024.9984111
GTGTAAC8400.024.875871
GTGTTAC11200.024.4469761
TATACTG7400.024.3269775
TCTGTCA36350.023.7727818
GTACGGA2151.9585455E-523.4595221
Produced by FastQC (version 0.11.5)