FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2727303
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11900.042.9575088
TCGCTCT12100.041.6524354
GTCTCGC12500.039.7610551
CTGTCGC15000.034.0796249
GTCTTGC27350.033.7109871
CGCTCTG14400.032.499645
TCTCGCT15600.031.384272
CTCGCTC15550.030.0961283
GTGTTAC10900.029.7375261
GTGTTAT11450.028.3090861
GGTATAC5650.028.0474261
CCGTTAT1555.7527315E-527.8829271
GTACGGA2654.8930815E-927.181471
CTTCGTA2456.895061E-826.4486879
GTTTCAC40450.025.987353
GTATAAG7900.025.5299381
GTCTTAC11300.024.860221
GTCTCAC33350.024.838241
TATACGG1450.001277573424.8273122
GGTTACG1500.001552520424.01031
Produced by FastQC (version 0.11.5)