FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2727303
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12750.043.4857528
GTCTCGC12550.040.7773741
CTGTCGC15200.036.9489029
TCGCTCT15550.033.800194
GTCTTGC26700.032.934661
TCTCGCT16550.030.025982
CTCGCTC17200.029.3019283
CGCTCTG17850.027.8315565
GTGTTAG19000.025.0377311
TAATACT8400.024.8568084
GTGTTAC11000.024.2445241
GTTTCAC39600.024.1814943
GTATTAT9400.023.770511
GTATTAG10050.023.6675071
GGGTTTC45150.023.3077161
TTGCTCT36350.023.1743774
GTACCGG2802.48152E-723.1680321
CTTGCTC33550.022.747833
GGGTAAT9250.022.5975271
GTACGAA1600.002251135222.5244751
Produced by FastQC (version 0.11.5)