FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2614077
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11800.040.270878
TCGCTCT12550.039.0123944
GTCTCGC12050.038.838611
GTCTTGC24300.034.963361
TCTCGCT14050.034.847372
CTCGCTC13850.033.7909933
CTGTCGC14150.033.582789
TCTATCG1652.520952E-630.5452188
CGCTCTG14800.028.7030265
TTGCTCT34600.026.8442334
GTATTAG8300.026.024391
GTGTAAA16150.025.412051
CTTGCTC32500.024.8125883
GTATAGA6600.024.0002731
GTATTAT6900.024.000271
GTTTCAC39550.023.3024143
GTCGTAC1550.001883461423.2260681
GGGTTTC41900.023.0265121
GTCTCAC30200.022.8876761
ATAGTAC6350.022.6774223
Produced by FastQC (version 0.11.5)