FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2978038
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11700.042.461278
GTCTCGC13700.041.518681
CTGTCGC13700.037.8391769
GTCTTGC29450.033.249921
TCGCTCT14300.033.231124
TCTCGCT17350.029.4642872
GTACGCG1255.3690426E-428.8003031
GTCTTAC12250.027.624781
CTCGCTC17750.026.7721123
CGCTCTG16200.026.6669465
TAATACT8050.025.9381624
GTGTAGT17800.025.887911
GTGTTAG21650.025.2750931
GTCTCAC37050.025.2634221
TACACTG18200.024.923345
TTCACCG9450.024.3812085
GTATTAT8600.024.2793251
GTGTAAC9250.024.1299841
ACCGTGT10450.024.1146818
GTATTAG10600.023.7738361
Produced by FastQC (version 0.11.5)