FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2978038
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11550.039.89548
CTGTCGC15500.032.980069
GTCTCGC15250.032.1707881
GTCTTGC25800.032.158871
TCGCTCT15150.030.8905454
GTATAAC5850.028.3657741
GGTATAC5700.027.846491
GTATTAG9650.027.6628591
TCTCGCT17450.027.6442152
GTGTTAC12750.025.4639051
GTGTAAC9850.024.9037841
GTATTAA8150.024.786951
GTGTAAT11200.024.4786931
GTACTAG5650.024.2620681
CTCGCTC18200.024.1314433
GTGTTAG18600.024.0492441
GTCTCAC35600.023.9141331
GTATAAT8450.023.9069391
GTATTAT9100.023.7849641
CGCTCTG17650.023.6596415
Produced by FastQC (version 0.11.5)