FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3368803
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15450.043.3418358
GTCTCGC13850.041.313981
CTGTCGC17850.037.5143629
TCGCTCT16200.035.5545854
CTCGCTC17850.032.6699183
TCTCGCT18550.031.0549722
CGCTCTG17200.030.5573045
GTCTTGC33050.030.0240171
GTGTTAC14800.027.4060231
CTCGATC13350.026.4282048
GTACTAT7600.025.7317831
GTATTAC7750.025.2337471
GTATTAG10650.024.4834481
GTCTTAC14000.024.3158531
TCTCGAT13150.023.543087
GTGTTAG22500.023.4995771
GTTAGCC18100.023.4679243
TTGCTCT42250.023.1756954
TCGATCT13750.023.0410779
GTTTCAC48900.021.9370733
Produced by FastQC (version 0.11.5)