FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2114686
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT41350.19553730435629685No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8550.038.736728
GTCTCGC10100.035.6451381
TCGCTCT11450.032.0712854
TCTCGCT12200.028.3291192
CTGTCGC11450.027.6680369
GGCGTAC1658.8261775E-526.1829761
GTCTTGC21000.025.7154221
CTCGCTC12100.025.587913
CGCTCTG12350.025.0699335
GTCTTAG8300.024.290231
TGTAACG1550.001882982223.2268332
GTCTAGC7800.022.1548251
GTCTTAC6850.022.0739671
GTCTCAC23200.021.7250981
GTACTAC3157.7873847E-720.5723381
GTACTGA6750.020.2675631
GTGTTAC8250.020.0736141
GTGTTAG13350.019.9559381
GAGTGCA54900.019.4106926
TAATACT5204.9112714E-1119.3854714
Produced by FastQC (version 0.11.5)