FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3158886
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13800.041.7389538
GTCTCGC14300.038.7690661
CTGTCGC16550.037.413749
GTCTTGC29650.036.6676371
CGTTTCG1406.9841553E-735.9998479
TCGCTCT16050.034.093314
CTCGCTC17400.029.3791853
TCTCGCT18350.029.0352972
GTCTTAC12300.027.5120771
CGCTCTG18500.027.2431285
GTATTAC7700.027.1167661
GTACGGT2508.386087E-825.919891
GTGTAAT13150.025.733731
GTCTCAC38150.025.6669921
TTGCTCT40250.025.0433714
GTATAGG8350.025.0058821
GTGTTAG23400.024.9229721
TACCCCG2901.2838427E-824.8274785
GGGTTTC52050.023.7924061
GTGTAAA19050.023.432971
Produced by FastQC (version 0.11.5)