FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3064591
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11950.046.9956328
GTCTCGC13900.039.3673931
TCGCTCT15050.036.3592534
CTGTCGC16750.035.677479
TCTCGCT16500.031.8549392
CGCTCTG16850.029.0567365
CTCGCTC17550.028.7183023
GTCTTGC28750.028.5499171
GTACTAC6300.026.2860371
GTTTCAC47550.025.8930633
GTGTAAC8950.025.7433341
GGGTTTC52250.025.0797351
GTACTAG6050.024.9920441
GTCTCAC37300.024.9011121
GGTATAC5950.024.2019791
GTATTAG10850.023.8896961
GTTAGCC17300.023.7228363
GTGTAAA16800.023.571721
GTGTTAA12100.023.2068981
GTGTAAG13350.023.1912981
Produced by FastQC (version 0.11.5)