FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3064591
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13450.042.8245858
CTGTCGC17550.034.462679
GTCTCGC14000.034.0051
GTCTTGC28000.032.716931
TCGCTCT15900.028.5278384
GTCTTAC11650.027.2429181
TTATACT7550.026.7015554
GTATCGC1100.00991770226.2297531
GGGTTTC50350.025.2139021
GTGTAAC8450.024.7552991
TTGCTCT40050.024.6288154
TCTCGCT17550.024.615792
GTGTAAA16550.024.407141
CTCGCTC17200.024.2786753
GTATTAG8750.023.9065481
GTTTCAC46400.023.5858233
GTTAGCC16850.023.5011043
TAACGGT1851.9200364E-423.3509714
GTATAGG6500.023.3041271
GTGTTAG22600.023.2992151
Produced by FastQC (version 0.11.5)