FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2843008
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12000.047.99978
GTCTCGC13350.047.4612121
TCGCTCT15000.041.2804684
CTGTCGC16050.039.0277949
TCTCGCT15900.037.5853352
CTCGCTC17500.034.5603943
CGCTCTG17150.033.166565
GTCTTGC27650.030.9876941
ACCGTGT9200.028.1737378
TTCACCG8300.027.7593525
GGGTTTC48650.027.3795491
GTTTCAC43450.026.8449543
TACACTG18350.026.6815035
GTATTAG9550.025.6337991
TTACACT14500.025.3244274
GTCTTAC10600.025.132361
GGTATAC5450.025.1012021
GTATTAC7850.024.7646121
GTATTAT7500.024.0002731
GTATTAA8150.023.853031
Produced by FastQC (version 0.11.5)