FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2843008
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11650.038.9349758
TCGCTCT13800.038.086324
GTCTCGC13150.037.8500631
GTCTTGC26700.032.1498531
GTATTAG9200.032.1469151
CGCTCTG15700.032.101375
CTGTCGC14400.031.000579
CTCGCTC15600.030.4610273
TCTCGCT16350.028.624382
GTATTAT8650.026.6856121
GTATAGC6400.025.923351
GTCTTAC12250.025.3206961
TTGCTCT35800.025.1392444
GTCTCAC34650.024.9816591
GTGTTAT12200.024.8332021
GTATAGA8800.024.591321
GTATAGG6750.024.5791761
CTTGCTC34550.024.3816473
GTGTTAG17750.023.9771141
GAGTGCA74800.023.4862386
Produced by FastQC (version 0.11.5)