FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2714224
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10250.035.824268
GTATAAC5150.033.5532761
GTCTCGC12300.031.6096421
CTGTCGC13050.029.7929959
GTATTAG9250.029.5782721
GTATCGC1504.6142482E-528.7998961
GTCTTGC24150.028.6210161
TCGCTCT14650.026.047694
CGCTCTG16050.025.1214075
GTGTTAG21400.024.8971081
TCTCGCT15300.023.9999142
GTGTAAC8450.023.8579041
TCGATCT11850.023.6961179
GTGTAAG12800.023.6249161
ACCGTGT8850.023.5931368
CTCGATC11950.022.8953158
GTATAAG7600.022.7367611
GTCTCAC32050.022.4648171
GTTCTAC9050.022.2761631
TTGCTCT32900.022.1032644
Produced by FastQC (version 0.11.5)