FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2915271
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11850.042.531498
GTCTCGC13450.040.6845631
CTGTCGC14650.034.894079
TCGCTCT13750.034.0368234
GGCGTAC1352.247256E-532.000431
TCTCGCT15850.030.8900052
GTCTTGC29500.030.7529561
GCGTACT950.004891233530.31622
CTCGCTC14950.029.3783233
CGCTCTG16100.028.6215025
GTATTAT11200.027.643231
GTATAAC5850.027.0772861
GTATTAG9750.026.5849741
GTATTAA10200.025.4121071
TTCACCG8050.025.0438145
GTACGGG2301.1728407E-625.0438141
GTGTTAT12700.024.9452191
GTATTAC7850.024.7646641
GTCTTAC12400.024.3874261
GTGTAAC9400.023.7451
Produced by FastQC (version 0.11.5)