FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3421888
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15400.047.2252248
CTGTCGC16350.044.9216549
GTCTCGC16550.042.4521561
TCGCTCT17500.037.8511164
CTCGCTC19400.034.5126573
TCTCGCT20550.033.987112
GTCTTGC32800.033.5657041
CGCTCTG19150.031.5819845
GGGTTTC55000.027.3921831
GTTTCAC48800.025.2274593
TTGCTCT43800.024.8217144
ACCGTGT10600.024.4551328
GTGTAAA19900.024.0224271
GTATTAG11550.023.8301941
GTATAAT10450.023.5661491
CCGTGTT11650.023.4871889
TTCACCG10150.022.699325
GTCTCAC38650.022.675761
TAATACT10500.022.6283844
CTTGCTC42050.022.4286163
Produced by FastQC (version 0.11.5)