FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3421888
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13000.044.301918
GTCTCGC14300.038.4816171
GTCTTGC30500.034.881591
CTGTCGC17050.034.6230439
TCGCTCT16700.031.9000284
CGCTCTG18100.029.4326235
TCTCGCT18500.029.1853792
GTATAGA9650.028.1321221
GGTATAC6950.026.3926681
TTGCTCT42300.025.6987744
CTCGCTC20300.025.5336133
GTTTCAC52000.025.3351543
CTGTTGC36450.025.0831459
GGGTTTC53900.025.0470811
GTCTCAC41050.025.0232071
GTATTGA9850.024.5813521
GTATTAG11400.024.4572071
GTGTTAG24150.024.3052961
ACCGTGT10700.024.221148
GTGTTAC12750.024.1694741
Produced by FastQC (version 0.11.5)