FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2917880
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAC1651.3405952E-939.624571
GTCTCGC10400.034.9255031
CCGATCG950.00472935530.525549
TCTGTCG11450.030.3922378
CGCTATA1000.006062866729.013182
TCGCTCT12050.028.8883384
GTCTTGC27800.028.7444421
CTGTCGC14400.026.6833489
TTACTCG2001.0639031E-525.3826164
TCTCGCT13550.024.6237322
GTATAAT9150.024.611981
GCGTTAA1500.001477659424.2150141
GTATAGA8250.023.774741
GTATTAA9750.023.0973991
GTGTACG3152.809611E-823.0619181
GTATAAG9200.022.8989811
GTATAGT7950.022.8443531
CGCTCTG14200.022.4638295
GTGCTAA12100.022.2137741
GTATTAC6550.022.1816921
Produced by FastQC (version 0.11.5)