FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2707403
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG8200.041.635641
TCTGTCG10800.035.5780648
GTACTAG5650.030.8563121
GTCTCGC11850.030.6501711
TCGCTCT12800.030.028424
GTCTTGC24350.029.8319911
GTATAGG6300.028.8257541
CTGTCGC13950.027.544319
GTGTTAC9700.026.9595091
GTCTCAC31950.026.6008951
TCTCGCT13300.025.0867842
GTGTATA11900.024.417111
GTGTTAG16850.024.1417851
GTGTAGA12350.024.1156041
TACCCCG2751.9718937E-723.726415
CTCGCTC14400.023.67023
GTATAGA6600.023.1130141
TATACTG7600.022.8939025
TTATACT8450.022.3142954
GTATTAT7550.022.1290171
Produced by FastQC (version 0.11.5)