FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2668882
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11250.044.4661758
GTCTCGC10150.042.4317861
TCGCTCT12050.039.7046434
CTGTCGC14050.037.152629
GGTATAC5600.035.195011
CGCTCTG15600.031.136315
TCTCGCT14250.030.0166512
CTCGCTC15150.027.7533913
CTACGCG1050.007711130727.6187469
GTCTTGC26150.026.7981551
GTATAGG7450.026.4553091
TAAGTCG1658.426515E-526.362365
GTGTAAC7100.025.7031881
GTGTAAA15700.023.712421
TAATACT8300.023.5814274
GTATTAA9000.023.5212751
GTATTAC6550.023.4036351
GTCTTAC11650.022.5570321
GATCGAT1952.6165243E-422.3074476
GTATAGC5900.022.2702881
Produced by FastQC (version 0.11.5)