FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2719468
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGT2900.037.75561
TCTGTCG10950.031.7809228
GTCTTGC25550.029.99761
GTCTCGC11400.028.1731851
GGTATAC7100.026.7302551
GTCTCAC30650.026.435081
GTCTTAC11250.025.9534781
CGTAGTG3108.60382E-1025.7258872
GTGTAAA15850.025.3292031
TCGCTCT12100.025.163984
GTATTAG10100.024.572291
CTGTCGC16050.023.9408869
GGAGTGC56100.023.7781685
GTGTAAG14850.023.5940721
GTATTAC6250.023.358131
GTACAGG13450.022.7937151
TTACACT13450.022.0992224
GTGTTAC10900.022.099151
GGGTACG2002.9655674E-421.8982471
TCTCGCT13600.021.8566862
Produced by FastQC (version 0.11.5)