FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2906718
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11350.046.6283048
GTCTCGC12050.040.3945471
CTGTCGC14400.037.2562649
GTCTTGC26700.032.9237331
TCGCTCT12600.032.8116954
TCTCGCT14950.030.570742
GTCTTAC11600.030.0620271
GTATTAC7950.029.2427231
CTCGCTC15050.027.9521883
CGCTCTG13950.027.0236665
GTCTCAC34100.026.6311911
GTATAGA8500.025.6411381
GGTATAC6600.025.3173871
GTGTTAG18700.024.8641341
TCTGTCA37300.024.684118
GTGTATA15150.024.4563981
GTGTTAC11700.024.216631
GTATAAG8450.024.0733381
GTATTAA10000.023.9744641
TTGCTCT37200.023.592114
Produced by FastQC (version 0.11.5)