FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2906718
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10700.046.0747958
TCGCTCT12950.036.3868524
GTCTCGC11750.036.0233731
CTGTCGC14450.035.1211289
GTCTTGC25650.032.150321
GTATTAT9100.032.078411
CGCTCTG13400.031.9216615
CTCGCTC14800.029.3898853
GTATAAT9400.027.1728021
GACCGGG19050.025.87839145
TCTCGCT16350.025.2761152
TTACACT16100.025.2152394
GTCTCAC34350.024.21991
TATTATA9400.023.9104122
CTTTGCG4550.023.9011149
TTGCTCT36900.023.771684
GTGTAAC8000.023.7179721
GCGTTAC1550.001741389223.5414141
GTGTAAT11200.022.8057441
GTATAGG8000.022.8057421
Produced by FastQC (version 0.11.5)