FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2907822
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11250.047.0453648
GTCTCGC11250.046.699661
TCGCTCT13400.033.5413934
CTGTCGC15450.032.8485379
TCTCGCT15200.031.9578632
GCGTAGC2202.1296728E-829.8506371
CGCTCTG14450.029.6016375
CTCGCTC14450.029.600113
GTCTTGC24200.028.6445481
GTGTAAG13350.026.7823431
GTATAGA9900.023.5856881
GGAGTGC56100.022.7447625
TACGCCA2552.7124097E-622.7428044
GACTACG2903.0925366E-722.645311
GTACTGC9100.022.4517611
GTGTTAG19000.022.2745111
TCGCCGG6200.022.21703145
GTCTCAC31950.022.1531051
GTATAGG7300.021.9904231
GTATAAG8300.021.978381
Produced by FastQC (version 0.11.5)