FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2770577
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12600.039.1988641
TCTGTCG10900.033.9223868
TCTCGCT14650.032.179782
TCGCTCT13000.031.2390024
GTATTAA9000.029.0532721
CGCTCTG13350.028.7815255
CTCGCTC14100.028.2870853
GTATAGG6550.027.722591
TAGTACG1358.0739066E-426.8589294
CTGTCGC14100.026.7378779
GTATAAT9050.026.485031
GTCTTGC23850.026.1905821
GTATTAT9550.025.0983771
GTGTAAC7950.024.6678751
GTATAAG8350.024.356041
GTATTAG8950.024.346321
GTCTTAC11150.023.4510751
GGTATAC5900.023.3903481
GGCGTAA2502.2814274E-623.2002538
GTACACG2202.1992168E-523.110561
Produced by FastQC (version 0.11.5)