FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2770577
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10550.041.5067831
GTCTTGC23550.040.2877241
TCTGTCG11250.034.7996758
GTATTAT8200.030.2611451
CTGTCGC14050.028.3805189
GTACCGG1555.260518E-528.251391
GTATTAC6850.027.7014851
GTATAAG7950.027.5406631
TCGCTCT12200.027.332854
GTACTAG5850.026.1989381
GTCTTAC10900.026.1130961
TTGCTCT34700.025.6958264
GTATACG2301.0422245E-625.3853071
GTGTTAT12500.024.5222051
ATCGAAC2101.5571257E-524.1651333
TCTCGCT15550.023.7787722
GGAGTGC55000.023.4634175
GGTATAC5600.023.4587421
CTTGCTC33900.023.0958793
CTCGCTC13850.023.0310273
Produced by FastQC (version 0.11.5)