FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2722427
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11650.039.2738151
TCTGTCG11150.037.063468
TCGCTCT11850.036.104344
GTATCGA1251.2657272E-534.800646
GTCTTGC24000.032.984031
CTCGCTC12700.032.5459483
TCTCGCT13850.030.3704952
TAATACT8250.029.8848254
CGCTCTG14900.029.6801175
GTATAAC5350.028.507161
CTGTCGC15850.027.902729
GTATTAT9000.026.6293091
GGACCGT2756.7739165E-926.3641196
GCGTTAC1701.0198843E-425.632491
ATAGTAC7300.024.8337843
GTGTTAG17550.024.8291931
GTGTTAC10450.024.3242911
GTGTAAA15600.024.2084621
GTATAAG7200.024.2084621
GTAATAC6400.023.7938693
Produced by FastQC (version 0.11.5)