FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2515652
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9900.037.348268
GTCTCGC11550.037.1089061
TCGCTCT12150.031.6397884
CTGTCGC12750.031.2743239
GTCTTGC23400.028.8718911
GTCTTAC11700.028.561441
TCTCGCT13800.028.3857542
CGCTCTG12650.028.081725
CTCGCTC14000.026.9407253
GTATAGA6800.026.7078691
GTACTAT5500.025.0956841
GTCTCAC29650.024.5009771
GTATTAC6550.024.399981
GGTATAC4600.023.6887191
GTGTATA12900.023.0888481
CTTAGCG2202.2306478E-523.0680439
GTATAAG8200.023.0339071
TTGCTCT30200.022.576414
GTGTTAG17700.022.573431
GTGTTAT10350.022.4604131
Produced by FastQC (version 0.11.5)