FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2752694
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11050.036.9962121
TCGCTCT11250.036.728744
GGTATAC4800.033.4591
TCTGTCG10950.033.1044928
CTCGCTC12800.028.8852063
GTGTTAC11200.027.3755461
GTCTTGC22200.027.2933371
GTATTAC6200.027.0811841
CGCTCTG14800.026.9421395
CTGTCGC14650.026.723129
GTATACG2301.0398962E-625.391811
GTATTCG2601.07231244E-725.2697351
TCTCGCT14350.025.2622972
GTCTTAC10750.025.1260811
GGGTACG2351.2542423E-624.8515591
GGAGTGC60050.024.6290825
GAGTGCA66200.024.0932676
GTCTCAC33200.023.7474611
TAACACT13650.023.3670434
GTATATA10100.023.1291731
Produced by FastQC (version 0.11.5)