FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2879769
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10900.040.5722168
GTCTCGC12950.038.148541
CTGTCGC14300.033.4605649
TCGCTCT14200.032.6887254
TCTCGCT16650.030.933282
GGTATAC5800.028.809691
CGCTCTG14900.028.7072185
GTCTTGC28100.027.6640761
CTCGCTC17250.027.3289593
GTACGGT2051.2707693E-524.8074971
GCGTAGA2651.3508361E-724.6737611
GGGTAAT10350.023.8658731
ATACCCG2151.8617242E-523.6138044
GTGTAGT14400.022.7032871
GTATAGA8450.022.3540081
GTCTTAC11750.022.2588841
AGTACCG1650.00260247521.9690595
GTACGAT2003.061767E-421.7951561
TTACACT13000.021.7584344
GTGTAAT12800.021.5681251
Produced by FastQC (version 0.11.5)