FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2879769
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10050.050.1119731
TCGCTCT11950.044.8906944
TACACCG1752.4338078E-937.2850465
TCTCGCT13150.035.838972
TCTGTCG11700.035.320498
CTCGCTC13700.034.9251673
CGCTCTG14200.032.675475
GTCTTGC26900.032.560231
CTGTCGC15700.029.0923399
AATCCGT2104.991962E-727.6190326
GTATTAG10100.026.0159451
GGTATAC5650.025.8368761
GTGTAAG16050.025.466631
GTATAAG7950.024.7887761
GTGTAAT10500.022.9394551
GTATATA13750.022.8257051
GTGTTAT10250.022.7868651
TTGCTCT37800.022.4381244
GTGTAAA16600.022.4243851
GTATAAA13050.022.3721621
Produced by FastQC (version 0.11.5)