FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1788536
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC6450.039.6040341
TCTGTCG7000.035.214758
GTCTTGC14400.034.9717751
TTATACT4300.030.346694
ACCGTGT6650.028.3462378
TCGCTCT8600.027.8177994
GGATAAT7100.027.7546981
CCGTGTT6700.027.0525939
GTATTCG1357.7766145E-427.0313241
GTATAAG3850.026.5398451
TATACTT4100.026.5239965
GTACCGC1100.00967428326.3632476
TAGCGTC1100.0096769226.361774
GTGTAAC5050.026.0143071
GTATAGG4151.8189894E-1224.6213041
GTCTTAC5950.024.532631
GTCTCAC20600.024.4462911
CTCGCTC9200.024.4276183
CGCTCTG9500.024.420695
GTATAGA4500.024.3281921
Produced by FastQC (version 0.11.5)