FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2787440
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAC6300.033.4329531
TCTGTCG11400.033.0707328
GTACTAG4900.032.609481
GTCTCGC12500.030.795211
CTGTCGC14250.030.0185899
GTCTTGC27450.028.8404121
TCGCTCT12600.027.628934
GTCTTAC11250.027.1152781
CGCTCTG14450.026.0890035
GTATAGC7000.025.939361
TCTCGCT14650.025.7439212
GTGTTAG18950.025.6792811
GTGTTAC12100.025.2104851
GTATTAG8500.024.7797181
GTGTAAT10700.024.4363331
GTATAAG8350.023.4852161
GTATTAC6500.023.4651431
GTGTAGC12800.023.2643641
GTCTCAC30300.022.5321451
GTATACT5200.022.3477571
Produced by FastQC (version 0.11.5)