FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2787440
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10550.036.4206668
GTCTCGC12050.036.339011
TCGCTCT13400.030.8374844
CTGTCGC13450.030.7239489
GTCTTGC25550.028.849571
CTCGCTC14650.027.7124463
GTGTAAG14250.025.6073131
TCTCGCT15350.025.505922
GTATTAT8150.025.0731741
GTGTAAC7800.024.3269481
GTGTTAC11150.024.2178571
GTATTAA6950.024.1519321
GTGTAAA16050.024.099591
TATAGTA7050.023.6534422
GTATTAG7750.023.5422061
CGCTCTG15350.022.6703035
TTGCTCT35200.022.654734
GAGTGCA66350.022.2901866
GGAGTGC60750.022.0775035
GTATAAT8350.021.850551
Produced by FastQC (version 0.11.5)