FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3018306
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11100.037.484891
TCTGTCG11450.036.091638
GTCTTGC25050.032.0545231
CTGTCGC15350.028.8110149
TCGCTCT14050.027.8624044
GTGTTAC12650.027.6984231
GTATAGG8800.027.3738331
GTACGAT2155.7763464E-727.1616331
GTCCTAT7300.025.998891
GTATTAA9950.025.6772981
GTATTAG10750.024.445471
GTGTTAG19250.023.8898931
GTGTAAG13850.023.71741
TCTCGCT15650.023.6269322
GCGTAAC1550.001738837223.5473821
CTCGCTC16450.023.3574563
GTATTAC6900.023.274371
TTGCTCT35800.022.679644
GTATAAG8400.022.5942761
GTTCGGG2953.6326855E-722.2702391
Produced by FastQC (version 0.11.5)