FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2354711
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11050.039.447341
GTCGTAA1001.3753287E-436.3244251
GTCTTGC19600.032.2471921
TCGCTCT11350.031.3091874
TCTGTCG9350.031.0157038
TCTCGCT12400.031.0020452
GTAGTAC3900.029.7527393
TAATACT6300.028.778694
GTATTAG7550.027.9048541
GTGTAAC5900.027.08941
GTCTTAC8400.026.8108831
TACGACA1658.405722E-526.3717464
CGCTCTG12100.025.7636345
CTCGCTC13250.025.7248943
GTGTATA10450.025.7225551
CTGTCGC13300.025.0749139
GTACTAG3801.2732926E-1124.8535521
ACCGTGT7600.024.8023598
GTCTCAC26400.024.2162821
GTGTTAG14750.024.1341931
Produced by FastQC (version 0.11.5)